The DeFi landscape continues to evolve with blockchain ecosystems competing for dominance through innovative applications and strategic partnerships. Total Value Locked (TVL) is one of the key metrics to measure the health and attractiveness of these ecosystems. In this article, we have compiled a list of the top 10 blockchains in September 2025 by TVL growth, with a minimum Range of $10 million, using data from DefiLlama, a leading DeFi analytics platform. 

What is TVL?

Total Value Locked (TVL) is a key metric in the decentralized finance (DeFi) ecosystem that represents the total U.S. dollar value of digital assets that are currently locked in DeFi platforms. These assets can include cryptocurrencies, stablecoins, liquidity tokens, and other digital assets used for various purposes such as staking, liquidity provision, and yield farming. It serves as an indicator of a DeFi platform's health and popularity, reflecting user trust and engagement.

Top 10 Blockchains in September 2025 by TVL Growth

The Following chains saw the highest TVL growth in September 2025

1. NEO

2. ENI

3. GOAT

4. Botanix

5. X Layer

6. Movement

7. Starknet

8. Moonbeam

9. Berachain

10. opBNB

1. NEO

NEO tops the table with 935% growth in September 2025. Neo is an open-source, community-driven blockchain platform designed for the smart economy by digitizing assets, identities, and enabling smart contracts. It employs delegated Byzantine Fault Tolerance (dBFT) for fast, secure transactions and supports decentralized apps and interoperability. Neo integrates digital identity verification for regulatory compliance and provides a scalable, efficient infrastructure for the next-generation Internet. Its TVL is now valued at $11.22 million.

Top protocol that drives TVL Growth for NEO:

Protocol	TVL Growth (Sep 2025)
NeoBurger	+4.08%

2. ENI

ENI comes on second place with 196% growth in September 2025. ENI Blockchain is a high-performance, enterprise-grade Web3 blockchain boasting 10,000+ TPS and multi-chain modularity. It offers one-click AppChain deployment, a triple-stacked consensus algorithm, full compatibility, and privacy-focused efficiency for scalable business applications. Its TVL is now valued at $30.37 million.

Top protocol that drives TVL Growth for ENI:

Protocol	TVL Growth (Sep 2025)
DAOaaS	+192%

3. GOAT

GOAT comes in third place with 157% growth in September 2025. GOAT Blockchain is a Bitcoin Layer 2 network offering trustless infrastructure, zero-trust bridges, and native BTC yield. Users earn real BTC rewards via SafeBox, staking, and BTCFi strategies, secure, scalable, and decentralizeds. Its TVL is now valued at $235.33 million.

Top protocols that drive TVL Growth for GOAT:

Protocols	TVL Growth (Sep 2025)
Pell Network	+160%
Stable Jack	+72%
Symbiosis	+11.01%

4. Botanix

Botanix comes in fourth place with 116% growth in September 2025. Botanix is a Bitcoin-based blockchain enabling secure, decentralized transactions and liquid Bitcoin yield via Spiderchain technology, supporting permissionless financial apps and on-chain usage for global users in a censorship-resistant ecosystem. Its TVL is valued at $19.93 million.

Top protocols that drive TVL Growth for Botanix:

Protocols	TVL Growth (Sep 2025)
Botanix stBTC	+170%
Dolomite	+116%
Palladium Protocol	+58.41%

5. X Layer

X Layer comes in fifth place with 81.29% growth in September 2025. X Layer is an EVM-compatible Layer 2 blockchain by OKX offering fast, low-cost transactions with an average cost of $0.0055 and 400 ms block time. It supports high throughput (1,000 TPS) and a large community, making it ideal for building and interacting with Web3 applications. Its TVL is now valued at $29.77 million.

Top protocols that drive TVL Growth for X Layer:

Protocols	TVL Growth (Sep 2025)
OkieSwap	+462%
PotatoSwap	+142%
DackieSwap	+82.02%

6. Movement

Movement comes in sixth place with 76.84% growth in September 2025. Movement is a next-generation network powered by Move and MoveVM, focusing on enhancing security, performance, and user experience in decentralized systems. It fosters a global Move developer community and uniquely settles to Ethereum, supporting DeFi, gaming, and NFTs. Its TVL is now valued at $291.56 million.

Top protocols that drive TVL Growth for Movement:

Protocols	TVL Growth (Sep 2025)
Avalon Labs	+120%
LayerBank	+0.05%

7. Starknet

Starknet comes at seventh with 76.77% growth in September 2025. Starknet is a Layer 2 Validity Rollup leveraging STARK cryptography to scale Ethereum and Bitcoin with high throughput, low fees, and strong security, enabling custom smart accounts and advanced DeFi apps with full decentralization in 2025. Its TVL is now valued at $213.38 million.

Top protocols that drive TVL Growth for Starknet:

Protocols	TVL Growth (Sep 2025)
Troves	+1187%
Endur	+913%
Ekubo	+252%

8. Moonbeam

Moonbeam comes in eighth place with 74.47% growth in September 2025. Moonbeam is a smart contract platform and Polkadot parachain enabling seamless cross-chain interoperability. It supports Ethereum-compatible dApps with EVM, Solidity, and tools like Metamask. It offers multi-chain integration, high scalability, and a growing DeFi ecosystem fueled by its GLMR token. Its TVL is now valued at $12.46 million.

Top protocols that drive TVL Growth for Moonbeam:

Protocols	TVL Growth (Sep 2025)
Balmy	+1547%
1Beam	+742%
Pickle	+467%

9. Berachain

Berachain comes at ninth with 44.69% growth in September 2025. Berachain is a high-performance Layer 1 blockchain, EVM-identical for maximum Ethereum compatibility, using innovative Proof-of-Liquidity consensus and a tri-token model (BERA, BGT, HONEY) for secure, efficient, and liquidity-rich DeFi applications. Its TVL is now valued at $441.94 million.

Top protocols that drive TVL Growth for Berachain:

Protocols	TVL Growth (Sep 2025)
Stride	+2619%
WinnieSwap	+816%
Concrete	+246%

10. opBNB

opBNB comes at tenth with 42.19% growth in September 2025. opBNB is a high-performance Layer 2 scaling solution for the BNB Smart Chain, built on the OP Stack using Optimistic Rollup technology. It enhances scalability, enabling up to 5,000+ TPS, lowers transaction fees, and ensures security by anchoring to BNB Chain's mainnet.  Its TVL is now valued at $19.69 million.

Top protocols that drive TVL Growth for Cronos opBNB:

Protocol	TVL Growth (Sep 2025)
PancakeSwap	+49.21%
PixelSwap	+41.40%
White Protocol	+41.20%

Conclusion

In the dynamic landscape of decentralized finance (DeFi), tracking the Total Value Locked (TVL) across various blockchains provides invaluable insights into market trends and investor confidence. This analysis, based on data from DeFi Llama, highlights the top 10 blockchains that have experienced significant growth in TVL over September month, with a minimum TVL of $10 million.